7DKG
Influenza H5N1 nucleoprotein (truncated) in complex with nucleotides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.810, 60.120, 81.370 |
| Unit cell angles | 107.31, 106.68, 96.11 |
Refinement procedure
| Resolution | 36.610 - 3.000 |
| R-factor | 0.29085 |
| Rwork | 0.289 |
| R-free | 0.33167 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | An in-house model not deposited |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.316 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.580 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.168 | 0.171 |
| Number of reflections | 15710 | 2610 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 86.1 | 88.2 |
| Redundancy | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M HEPES, pH 7.5 and 5% propan-2-ol |
