7DKG
Influenza H5N1 nucleoprotein (truncated) in complex with nucleotides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 |
Unit cell lengths | 53.810, 60.120, 81.370 |
Unit cell angles | 107.31, 106.68, 96.11 |
Refinement procedure
Resolution | 36.610 - 3.000 |
R-factor | 0.29085 |
Rwork | 0.289 |
R-free | 0.33167 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | An in-house model not deposited |
RMSD bond length | 0.007 |
RMSD bond angle | 1.316 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.580 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.168 | 0.171 |
Number of reflections | 15710 | 2610 |
<I/σ(I)> | 5.8 | |
Completeness [%] | 86.1 | 88.2 |
Redundancy | 1.9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M HEPES, pH 7.5 and 5% propan-2-ol |