7DKA

Crystal structure of DsbA-like protein DR2335 from Deinococcus radiodurans R1, C24S mutant protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 1 21 1
Unit cell lengths	45.084, 84.177, 63.898
Unit cell angles	90.00, 91.54, 90.00

Refinement procedure

Resolution	29.860 - 1.560
R-factor	0.1562
Rwork	0.155
R-free	0.18050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dk9
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.560	1.560
Rmerge	0.139	0.782
Rmeas	0.150	0.842
Rpim	0.055	0.310
Number of reflections	67292	3334
<I/σ(I)>	37.635	4.845
Completeness [%]	99.9
Redundancy	7.4
CC(1/2)	0.986	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	Ammonium acetate, Sodium acetate trihydrate pH 4.6, 30% w/v PEG 4000