7DK9

Crystal structure of DsbA-like protein DR2335 from Deinococcus radiodurans R1, native protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-25
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	45.475, 87.389, 63.414
Unit cell angles	90.00, 90.09, 90.00

Refinement procedure

Resolution	31.510 - 1.720
R-factor	0.1793
Rwork	0.178
R-free	0.22010
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.720	1.720
Rmerge	0.056	0.182
Rmeas	0.061	0.204
Rpim	0.024	0.089
Number of reflections	51343	2628
<I/σ(I)>	38.8	7.932
Completeness [%]	97.2
Redundancy	5.7
CC(1/2)		0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	Sodium potassium phosphate pH 7.0, 8% w/v PEG 500 MME