7DK8

Crystal structure of OsGH3-8 with AMP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.979183
Spacegroup name	P 63
Unit cell lengths	121.050, 121.050, 62.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.578 - 1.990
R-factor	0.2
Rwork	0.197
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b2g
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.520	2.040
High resolution limit [Å]	1.990	1.990
Rmerge	0.022	0.307
Number of reflections	35491	36047
<I/σ(I)>	20.3
Completeness [%]	98.4
Redundancy	19.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.08M Tris pH 8.0, 0.1M Tris-HCl (pH 8.5), 22.4% (w/v) PEG 4000, 0.02M Bis-Tris pH 6.5, 5% (w/v) PEG 3350, 0.2 Microliter 275.0mM 2,6-Dimethyl-4-heptyl-beta-D-maltopyranoside