7DJQ
Crystal Structure of O-acetyl L-serine sulfhydrylase from Haemophilus influenzae in complex with C-Terminal peptide of ribosomal S4 Domain protein from Lactobacillus salivarius.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2020-03-19 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.080, 98.940, 70.290 |
Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
Resolution | 40.860 - 2.300 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.22860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ho1 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.516 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.860 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.063 | 0.150 |
Number of reflections | 28307 | 2705 |
<I/σ(I)> | 17.1 | |
Completeness [%] | 97.7 | 94.98 |
Redundancy | 15.2 | 9 |
CC(1/2) | 0.855 | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 100mM HEPES buffer pH 7.4, 1.4M Sodium Citrate |