7DJM

Structure of four truncated and mutated forms of quenching protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-12-16
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9777
Spacegroup name	P 21 21 21
Unit cell lengths	55.000, 78.750, 103.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.375 - 1.700
R-factor	0.187504658017
Rwork	0.186
R-free	0.21133
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7djj
RMSD bond length	0.009
RMSD bond angle	1.015
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmeas	0.082
Number of reflections	49848	2367
<I/σ(I)>	33.36
Completeness [%]	99.8
Redundancy	12.4
CC(1/2)		0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	25-34% PEG 3350, 0.2 M NH4Ac, 0.1M MES, pH 5.9-6.6