7DJK
Structure of four truncated and mutated forms of quenching protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-03-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 183.191, 183.191, 120.894 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.840 - 2.801 |
R-factor | 0.179747306216 |
Rwork | 0.178 |
R-free | 0.21847 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gc1 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.223 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rpim | 0.050 | |
Number of reflections | 25154 | 1241 |
<I/σ(I)> | 12.13 | |
Completeness [%] | 98.5 | |
Redundancy | 5.2 | |
CC(1/2) | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291.15 | 0.1 M NaCl, 0.1 M tri-Sodium citrate pH 5.6, 12% (w/v) polyethylene glycol 4000 (PEG 4000) |