7DIG
Green fluorescent protein from Dendronephthya sp. SSAL-2002
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 115.826, 124.737, 129.059 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.700 - 2.600 |
R-factor | 0.2364 |
Rwork | 0.234 |
R-free | 0.28130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5exb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.634 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.136 | 0.357 |
Number of reflections | 55239 | 2523 |
<I/σ(I)> | 10.6 | 1.6 |
Completeness [%] | 95.5 | 89.3 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | Tris, MgCl2, PEG 4000 |