7DHP

Crystal structure of MazF from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2015-12-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	70.490, 70.490, 111.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.830 - 1.300
R-factor	0.1723
Rwork	0.172
R-free	0.18480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kys
RMSD bond length	0.018
RMSD bond angle	1.652
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.340
High resolution limit [Å]	1.300	5.830	1.300
Rmerge	0.059	0.037	0.516
Rmeas	0.068	0.045	0.590
Number of reflections	66360	338	4953
<I/σ(I)>	14.38	21.98	3.52
Completeness [%]	96.1	37.5	99
Redundancy	4.179	2.817	4.21
CC(1/2)	0.998	0.996	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	ammonium sulfate, BIS-TRIS, PEG 3350