7DHD
Vibrio vulnificus Wzb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9788 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.315, 48.741, 117.316 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.660 - 1.710 |
R-factor | 0.2 |
Rwork | 0.199 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wmy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | XDS (Jan 31, 2020 (BUILT 20200417)) |
Data scaling software | Aimless (0.5.29) |
Phasing software | PHASER (2.7.17) |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.660 | 1.740 |
High resolution limit [Å] | 1.710 | 1.711 |
Rmeas | 0.088 | 1.238 |
Rpim | 0.025 | 0.343 |
Number of reflections | 29957 | 1506 |
<I/σ(I)> | 19.2 | 2.3 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 12.6 | 12.9 |
CC(1/2) | 0.999 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Free VvWzb crystals were grown in drops containing 1.5 ul of protein solution (14 mg/ml in the buffer 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT) and 1.5 ul of reservoir solution (100 mM HEPES pH7.5, 200 mM NaCl, 31% (w/v) PEG 3350) |