7DH1
The structure of the Arabidopsis thaliana guanosine deaminase in reaction with N2-Methylguanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 118.776, 118.776, 39.540 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.350 - 1.850 |
R-factor | 0.165 |
Rwork | 0.163 |
R-free | 0.19870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dbf |
RMSD bond length | 0.014 |
RMSD bond angle | 1.252 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.120 | 0.739 |
Number of reflections | 27608 | 2600 |
<I/σ(I)> | 28 | 2.25 |
Completeness [%] | 99.6 | 95.6 |
Redundancy | 19.3 | 16.3 |
CC(1/2) | 0.994 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2M Na-citrate and 0.1M HEPES (pH 7.5) |