7DGU
De novo designed protein H4A1R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-20 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5406 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 33.407, 33.407, 159.787 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.810 - 1.750 |
R-factor | 0.2017 |
Rwork | 0.198 |
R-free | 0.23110 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.810 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.072 | 1.008 |
Number of reflections | 11152 | 1073 |
<I/σ(I)> | 18.4 | |
Completeness [%] | 99.7 | |
Redundancy | 9.8 | |
CC(1/2) | 0.999 | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.0 M Potassium/sodium phosphate pH 7.0 |