7DGC

The structure of the Arabidopsis thaliana guanosine deaminase in reaction with 2'-O-Methylguanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	119.052, 119.052, 39.809
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.510 - 2.100
R-factor	0.1847
Rwork	0.183
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.243	0.901
Number of reflections	19002	1824
<I/σ(I)>	19.14	2.14
Completeness [%]	99.9	98.8
Redundancy	16.9	10
CC(1/2)	0.981	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)