7DG9

DPBB domain of VCP-like ATPase from Aeropyrum pernix

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-31
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	26.040, 31.066, 94.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.078 - 1.602
R-factor	0.1728
Rwork	0.169
R-free	0.20680
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	19200	1569
<I/σ(I)>	33.41
Completeness [%]	98.8
Redundancy	7.21
CC(1/2)	0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM Sodium acetate pH4.5, 20% PEG 1000, 200mM Zinc acetate