7DG7
DPBB domain of VCP-like ATPase from Methanopyrus kandleri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-21 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.078, 29.787, 42.155 |
| Unit cell angles | 90.00, 106.42, 90.00 |
Refinement procedure
| Resolution | 40.436 - 1.604 |
| R-factor | 0.2086 |
| Rwork | 0.206 |
| R-free | 0.22730 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 10094 | 1569 |
| <I/σ(I)> | 22.97 | |
| Completeness [%] | 98.7 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.990 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM imidazole/HCl pH7.8, 2M NaCl, 200mM Zinc acetate |
