7DG5

Crystal structure of mouse Smc1-Smc3 hinge domain containing a D574Y mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97960
Spacegroup name	P 1
Unit cell lengths	50.080, 60.851, 77.757
Unit cell angles	72.66, 88.43, 89.99

Refinement procedure

Resolution	42.060 - 2.000
R-factor	0.2207
Rwork	0.219
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wd5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.064	0.049	0.176
Rmeas	0.078	0.059	0.225
Rpim	0.044	0.032	0.139
Total number of observations	134894
Number of reflections	52576	2859	2279
<I/σ(I)>	7.3
Completeness [%]	88.6	96.1	76.6
Redundancy	2.6	3.3	1.9
CC(1/2)		0.994	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	Sodium phosphate, Potassium phosphate, PEG 3350