7DG2

Nse1-Nse3-Nse4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	103
Detector technology	PIXEL
Collection date	2019-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	53.486, 66.161, 168.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.590 - 1.700
R-factor	0.1912
Rwork	0.190
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvq
RMSD bond length	0.006
RMSD bond angle	0.760
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.103
Number of reflections	65684	3086
<I/σ(I)>	23.7
Completeness [%]	98.7
Redundancy	12.3
CC(1/2)	0.996	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	Tris, PEG 3350