7DG0

Structure and function of Diadenylate cyclase DacM in Mycoplasma ovipneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	41.057, 68.683, 113.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.196 - 1.895
R-factor	0.1749
Rwork	0.173
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rv7
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.890	1.890
Rmerge	0.084	0.084
Number of reflections	24587	24587
<I/σ(I)>	28.1
Completeness [%]	99.9
Redundancy	12.4
CC(1/2)	0.995	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1 M HEPES pH 7.5, 19% (w/v) PEG 3350