7DFY
Novel motif for left-handed G-quadruplex formation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.405, 31.601, 77.764 |
Unit cell angles | 90.00, 98.07, 90.00 |
Refinement procedure
Resolution | 76.990 - 1.690 |
R-factor | 0.2039 |
Rwork | 0.201 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gz6 |
Data scaling software | XDS |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.995 | 1.904 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.189 | 1.212 |
Rmeas | 0.206 | |
Rpim | 0.080 | 0.739 |
Number of reflections | 9010 | 450 |
<I/σ(I)> | 8 | 1 |
Completeness [%] | 86.2 | 40.5 |
Redundancy | 6.3 | 3.5 |
CC(1/2) | 0.996 | 0.349 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.08 M Strontium Chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate ph=6.0, 35% v/v (+/-)-2-Methyl-2,4-pentanediol (MPD), 0.012 M Spermine tetrahydrochoride |