7DFX

Structure and function of Diadenylate cyclase DacM in Mycoplasma ovipneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	I 21 3
Unit cell lengths	192.075, 192.075, 192.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.941 - 2.996
R-factor	0.1703
Rwork	0.169
R-free	0.19190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rv7
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.990	3.000
Rmerge	0.208	0.208
Number of reflections	23720	23720
<I/σ(I)>	23
Completeness [%]	100.0
Redundancy	36.1
CC(1/2)	0.999	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	1 M sodium malonate pH 5.0, 0.1 M citric acid pH 3.5