7DFT

Crystal structure of Xanthomonas oryzae ClpP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9735
Spacegroup name	P 42 21 2
Unit cell lengths	132.548, 132.548, 187.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.639 - 1.800
R-factor	0.1883
Rwork	0.186
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sta
RMSD bond length	0.008
RMSD bond angle	0.881
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.791	3.880	1.800
Rmerge	0.091	0.052	0.531
Rmeas	0.094	0.053	0.562
Rpim	0.021	0.011	0.174
Number of reflections	154153	16224	14800
<I/σ(I)>	6.7
Completeness [%]	99.4	99.9	96.9
Redundancy	16.9	24	9.5
CC(1/2)		0.999	0.257

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Sodium acetate trihydrate, 3.0 M Sodium chloride