7DFK
Crystal structure of xylitol-bound glucose isomerase by serial millisecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 298.15 |
| Detector technology | PIXEL |
| Collection date | 2019-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 94.138, 99.936, 103.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.780 - 1.400 |
| R-factor | 0.2352 |
| Rwork | 0.235 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ck0 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.400 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 95564 | 9462 |
| <I/σ(I)> | 4.15 | |
| Completeness [%] | 100.0 | |
| Redundancy | 277.9 | |
| CC(1/2) | 0.952 | 0.597 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 293.5 | Tris-HCl, Ammonium sulfate, Magnesium sulfate |
