7DFJ

Crystal structure of glucose isomerase by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-06-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	I 2 2 2
Unit cell lengths	93.940, 99.600, 102.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.570 - 1.500
R-factor	0.2239
Rwork	0.223
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ck0
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.900	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	77277	7646
<I/σ(I)>	3.46	1.13
Completeness [%]	100.0
Redundancy	736.2
CC(1/2)	0.948	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293.5	Tris-HCl, Ammonium sulfate, Magnesium sulfate