7DFB

Crystal of Arrestin2-V2Rpp-6-7-Fab30 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9776
Spacegroup name	I 21 21 21
Unit cell lengths	115.480, 116.881, 143.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.360 - 3.280
R-factor	0.2344
Rwork	0.231
R-free	0.29050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jqi
RMSD bond length	0.010
RMSD bond angle	1.255
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.360	3.540
High resolution limit [Å]	3.280	3.280
Rmerge	0.094	0.929
Rpim	0.040	0.376
Number of reflections	30318	3074
<I/σ(I)>	14
Completeness [%]	99.5
Redundancy	6.5
CC(1/2)	0.999	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	0% PEG 3350, 0.1M Succinic acid, pH 6.5-7.5, 0.2mM DMSO