7DFA
Crystal of Arrestin2-V2Rpp-4-Fab30 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 116.901, 121.142, 144.504 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.420 - 2.540 |
R-factor | 0.2362 |
Rwork | 0.234 |
R-free | 0.28010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jqi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.119 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.420 | 2.650 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.076 | |
Rpim | 0.032 | |
Number of reflections | 34294 | 4154 |
<I/σ(I)> | 14.3 | |
Completeness [%] | 99.9 | |
Redundancy | 6.6 | |
CC(1/2) | 0.999 | 0.614 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 289 | 10% PEG 3350, 0.1M Succinic acid, pH 6.5-7.5, 0.2mM DMSO |