7DF9

Crystal of Arrestin2-V2Rpp-1-Fab30 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97736
Spacegroup name	I 21 21 21
Unit cell lengths	116.174, 121.536, 144.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.250 - 3.170
R-factor	0.2566
Rwork	0.255
R-free	0.28050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jqi
RMSD bond length	0.012
RMSD bond angle	1.368
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.520	3.390
High resolution limit [Å]	3.170	3.170
Rmerge		1.459
Rpim		0.875
Number of reflections	17687	3145
<I/σ(I)>	9.8
Completeness [%]	99.8
Redundancy	5.6
CC(1/2)	0.990	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	10% PEG 3350, 0.1M Succinic acid, pH 6.5-7.5, 0.2mM DMSO