7DF2
Crystal structure of a C2 domain protein from Ramazzottius varieornatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.916, 62.916, 100.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.680 - 1.700 |
| R-factor | 0.1765 |
| Rwork | 0.175 |
| R-free | 0.21230 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.031 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.700 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.091 | 1.290 |
| Number of reflections | 22431 | 1128 |
| <I/σ(I)> | 16.2 | 2.5 |
| Completeness [%] | 98.4 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30 mg per mL protein, 0.1 M calcium chloride dihydrate, 0.1 M Tris pH 6.5, 13% (w/v) polyethylene glycol monomethyl ether 2000 |
