7DEQ
Lysozyme-sugar complex in D2O
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.029, 77.029, 38.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.448 - 1.030 |
| R-factor | 0.1331 |
| Rwork | 0.133 |
| R-free | 0.14850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wld |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.448 | 1.056 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.065 | 1.257 |
| Number of reflections | 57373 | 3914 |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 | |
| CC(1/2) | 1.000 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 40mg/mL lysozyme containing 2.1mg/mL (GlcNAc)4, 0.7M NaCl in a buffer of 50mM sodium-acetate (pD4.5) |
