7DEK
Pseudomonas aeruginosa FK506-binding protein PaFkbA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-01-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.073, 94.223, 117.437 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.540 - 2.900 |
R-factor | 0.2493 |
Rwork | 0.246 |
R-free | 0.28070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q6u |
RMSD bond length | 0.012 |
RMSD bond angle | 1.590 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 19438 | 1904 |
<I/σ(I)> | 2.154 | |
Completeness [%] | 99.8 | |
Redundancy | 8.6 | |
CC(1/2) | 0.992 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | 0.1M Tris pH 8.0, 28% PEG 4000 |