7DEJ

Structure of human ORP3 ORD in apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 31 2 1
Unit cell lengths	95.912, 95.912, 188.897
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.440 - 2.700
R-factor	0.2353
Rwork	0.233
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4inq
RMSD bond length	0.012
RMSD bond angle	1.335
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.076	0.368
Number of reflections	28026	1409
<I/σ(I)>	35.1	6.1
Completeness [%]	98.8	100
Redundancy	4.5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1 M MES-NaOH pH 6.0, 25% PEG 1500, 0.1 M Magnesium chloride