7DE9

crystal structure of Arabidopsis RDM15 tudor domain in complex with an H3K4me1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	28.579, 36.963, 46.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.978 - 1.711
R-factor	0.1843
Rwork	0.183
R-free	0.20220
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.954
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.099	0.069	0.500
Rmeas	0.106	0.074	0.533
Rpim	0.036	0.026	0.182
Number of reflections	5630	629	545
<I/σ(I)>	5.9
Completeness [%]	99.9	99.8	99.8
Redundancy	8.7	8.1	9
CC(1/2)		0.992	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCl, pH 8.5, and 20% PEG 1000