7DE8
Crystal Structure of outer membrane protein PorB with G103K mutations from Neisseria meningitidis W135
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 63 |
| Unit cell lengths | 84.396, 84.396, 107.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.237 - 2.760 |
| Rwork | 0.213 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vy8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.466 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 2.810 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.077 | 0.840 |
| Number of reflections | 11223 | 792 |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, pH 6.5 31% Jeffamine M-600 |
