7DE7

Crystal structure of PDZD7 HHD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97891
Spacegroup name	P 1 21 1
Unit cell lengths	32.782, 75.005, 42.939
Unit cell angles	90.00, 103.10, 90.00

Refinement procedure

Resolution	37.500 - 1.490
R-factor	0.1784
Rwork	0.177
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fdd
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.500	37.500	1.580
High resolution limit [Å]	1.490	4.460	1.490
Rmerge	0.084	0.038	1.197
Rmeas	0.091	0.041	1.301
Number of reflections	32592	1266	5160
<I/σ(I)>	13.49	44.52	1.56
Completeness [%]	99.4	99.6	98.2
Redundancy	6.74	6.678	6.442
CC(1/2)	0.999	0.999	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Bis-Tris pH 6.5, 45% Polypropylene glycol P400