7DE4
Hybrid cluster protein (HCP) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 142.540, 142.540, 117.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.290 - 3.610 |
| R-factor | 0.207 |
| Rwork | 0.203 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w9m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 3.378 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.290 | 3.710 |
| High resolution limit [Å] | 3.610 | 3.610 |
| Rmerge | 0.159 | 0.975 |
| Number of reflections | 14324 | 13606 |
| <I/σ(I)> | 14.16 | 3.01 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 11.48 | 11.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 2.0M Sodium formate, 0.1M Sodium acetate |
