7DDY
Crystal structure of an acetyl xylan esterase AlAXEase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.870, 80.610, 82.060 |
| Unit cell angles | 90.00, 103.13, 90.00 |
Refinement procedure
| Resolution | 49.327 - 2.505 |
| R-factor | 0.1781 |
| Rwork | 0.175 |
| R-free | 0.23440 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.918 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.137 | 0.306 |
| Rmeas | 0.162 | 0.362 |
| Rpim | 0.086 | 0.192 |
| Number of reflections | 33141 | 3086 |
| <I/σ(I)> | 8.25 | 2.67 |
| Completeness [%] | 98.8 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15% PEG 3350 0.1 M succinic acid |
