7DDU
Elephant seal myoglobin esMb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.090, 29.350, 75.360 |
Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
Resolution | 19.820 - 1.900 |
R-factor | 0.1993 |
Rwork | 0.199 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yce |
RMSD bond length | 0.013 |
RMSD bond angle | 1.161 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (1.17rc5_3630) |
Refinement software | PHENIX (1.17rc5_3630) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.024 | 0.104 |
Number of reflections | 22841 | 3287 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 99.8 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 3.1M Ammonium sulfate, 5.5% (w/v) PEG 3350 |