7DDA
Envelope protein VP37 a crystal structure from White Spot Syndrome Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 89.382, 89.382, 99.046 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.740 - 2.510 |
R-factor | 0.2047 |
Rwork | 0.201 |
R-free | 0.23800 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.912 |
Data reduction software | XDS (Jan 31, 2020) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.18.2-3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.691 | 2.548 |
High resolution limit [Å] | 2.505 | 2.505 |
Rmerge | 0.085 | 0.327 |
Rmeas | 0.086 | 0.331 |
Rpim | 0.014 | 0.054 |
Number of reflections | 8198 | 410 |
<I/σ(I)> | 43.6 | 12.2 |
Completeness [%] | 96.2 | 100 |
Redundancy | 37.1 | 36.1 |
CC(1/2) | 1.000 | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.2 | 1.5 M Ammonium sulfate, 100 mM Bis-Tris pH 6.5, 100 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |