7DD0
Crystal structure of the N-terminal domain of TagH from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-24 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.735, 90.667, 91.213 |
Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
Resolution | 19.730 - 2.700 |
R-factor | 0.1886 |
Rwork | 0.186 |
R-free | 0.23490 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.771 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.735 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.123 | 0.495 |
Number of reflections | 32572 | 3161 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 98.8 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% polyethylene glycol (PEG) 3,000 and 0.1 M sodium citrate (pH 5.5) |