7DCY
Apo form of Mycoplasma genitalium RNase R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.652, 80.768, 176.476 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.550 - 1.972 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.19980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xgu |
RMSD bond length | 0.011 |
RMSD bond angle | 0.978 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2148) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.175 | 2.931 |
Number of reflections | 70690 | 3444 |
<I/σ(I)> | 18.6 | 1.1 |
Completeness [%] | 99.9 | |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | 100 mM KCl, 100 mM HEPES (pH 6.8), 15% PEG 5000MME |