7DCN
Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-12-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.916, 36.827, 48.670 |
Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
Resolution | 32.793 - 1.695 |
R-factor | 0.1716 |
Rwork | 0.168 |
R-free | 0.20740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dcm |
RMSD bond length | 0.006 |
RMSD bond angle | 0.798 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.793 | 1.756 |
High resolution limit [Å] | 1.695 | 1.695 |
Number of reflections | 17060 | 7447 |
<I/σ(I)> | 13.15 | |
Completeness [%] | 99.7 | |
Redundancy | 5.2 | |
CC(1/2) | 0.996 | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.15M (NH4)2SO4, 0.1M MES, pH 5.5, 20% (w/v) PEG 4000, 8% (v/v) 1,3-Butanediol |