7DCB

The structure of the Arabidopsis thaliana guanosine deaminase complexed with inosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2020-04-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	117.980, 117.980, 37.736
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.060 - 2.000
R-factor	0.1663
Rwork	0.165
R-free	0.19750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.004
RMSD bond angle	0.681
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.223	0.759
Number of reflections	20634	1963
<I/σ(I)>	19.2	2.5
Completeness [%]	99.5	97.5
Redundancy	18.6	13.8
CC(1/2)	0.967	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)