7DCA
The structure of the Arabidopsis thaliana guanosine deaminase bound by xanthosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-09-05 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 119.350, 119.350, 39.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.670 - 2.100 |
| R-factor | 0.1743 |
| Rwork | 0.172 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dbf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.711 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.672 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.170 | 0.806 |
| Number of reflections | 19223 | 2785 |
| <I/σ(I)> | 12.1 | 2.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 8.6 | 5.6 |
| CC(1/2) | 0.992 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |
