7DC9
The structure of the Arabidopsis thaliana guanosine deaminase mutant E82Q complex with guanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 119.812, 119.812, 39.884 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.780 - 1.700 |
| R-factor | 0.1635 |
| Rwork | 0.162 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dbf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.311 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.072 | 0.907 |
| Number of reflections | 36327 | 3620 |
| <I/σ(I)> | 34.3 | 2.2 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 19.5 | 17 |
| CC(1/2) | 1.000 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1.2M Na-citrate and 0.1M HEPES (pH 7.5) |
