7DC6
Giant panda MHC class I complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 173.677, 173.677, 84.346 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.680 |
| R-factor | 0.2675 |
| Rwork | 0.266 |
| R-free | 0.29110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e27 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.094 | 0.094 |
| Number of reflections | 34668 | 34668 |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% (w/v) polyethylene glycol 3350, 0.05M magnesium chloride hexahydrate, 0.1M HEPES (pH 7.5) |
