7DC6
Giant panda MHC class I complexes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97892 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 173.677, 173.677, 84.346 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.680 |
R-factor | 0.2675 |
Rwork | 0.266 |
R-free | 0.29110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e27 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.094 | 0.094 |
Number of reflections | 34668 | 34668 |
<I/σ(I)> | 18.2 | |
Completeness [%] | 99.1 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% (w/v) polyethylene glycol 3350, 0.05M magnesium chloride hexahydrate, 0.1M HEPES (pH 7.5) |