7DC4
Crystal structure of glycan-bound Pseudomonas taiwanensis lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-18 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.85 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.057, 57.862, 58.899 |
| Unit cell angles | 90.00, 96.95, 90.00 |
Refinement procedure
| Resolution | 34.380 - 0.950 |
| R-factor | 0.1363 |
| Rwork | 0.136 |
| R-free | 0.14770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7dc0 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.740 | 42.740 | 0.970 |
| High resolution limit [Å] | 0.950 | 5.200 | 0.950 |
| Rmerge | 0.050 | 0.046 | 0.499 |
| Total number of observations | 599075 | 2989 | 28340 |
| Number of reflections | 180042 | 1153 | 8818 |
| <I/σ(I)> | 11.1 | 21.4 | 2.2 |
| Completeness [%] | 100.0 | 99.5 | 99.9 |
| Redundancy | 3.3 | 2.6 | 3.2 |
| CC(1/2) | 0.994 | 0.959 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG MME 5000, Ammonium sulfate, Mes |
