7DBS
Crystal Structure Of Biotin Protein Ligase From Leishmania Major in complex with Biotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 277.15 |
| Detector technology | PIXEL |
| Collection date | 2018-07-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979507 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.918, 44.700, 54.472 |
| Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
| Resolution | 52.840 - 2.330 |
| R-factor | 0.2179 |
| Rwork | 0.217 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jhu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.668 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.840 | 2.372 |
| High resolution limit [Å] | 2.330 | 2.332 |
| Rmerge | 0.062 | 0.631 |
| Rpim | 0.027 | 0.258 |
| Number of reflections | 11509 | 567 |
| <I/σ(I)> | 13.2 | 2.4 |
| Completeness [%] | 98.1 | 100 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.996 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 19.5% PEG 3350, 200 mM Ammonium sulfate |
