7DBF
The structure of the Arabidopsis thaliana guanosine deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 119.117, 119.117, 39.453 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.610 - 1.900 |
| R-factor | 0.1677 |
| Rwork | 0.166 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a8n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.733 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.897 | 1.900 |
| Rmerge | 0.128 | 0.815 |
| Number of reflections | 25636 | 1273 |
| <I/σ(I)> | 30.9 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.4 | 9.7 |
| CC(1/2) | 0.989 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2 M Na-citrate and 0.1 M HEPES (pH 7.5) |
