7DBA

RYX in complex with tubulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	105.449, 158.876, 181.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.300 - 2.461
R-factor	0.1886
Rwork	0.188
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i55
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge	0.141	0.091	0.809
Rmeas	0.153	0.099	0.880
Rpim	0.059	0.039	0.343
Total number of observations	722222
Number of reflections	110366	5861	5451
<I/σ(I)>	3.5
Completeness [%]	99.9	99.5	99.6
Redundancy	6.5	6.1	6.4
CC(1/2)		0.991	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	277	6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7