7DB9

IC1 in complex with tubulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	105.124, 157.684, 182.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.871 - 2.845
R-factor	0.1869
Rwork	0.185
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i55
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.845	7.730	2.850
Rmerge	0.177	0.053	0.954
Rmeas	0.184	0.055	0.992
Rpim	0.050	0.016	0.269
Number of reflections	71664	3854	3512
<I/σ(I)>	3.5
Completeness [%]	100.0	99.8	100
Redundancy	13.3	12.3	13.3
CC(1/2)		0.998	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	277	6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7