7DB9
IC1 in complex with tubulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.124, 157.684, 182.116 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.871 - 2.845 |
R-factor | 0.1869 |
Rwork | 0.185 |
R-free | 0.23260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i55 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.845 | 7.730 | 2.850 |
Rmerge | 0.177 | 0.053 | 0.954 |
Rmeas | 0.184 | 0.055 | 0.992 |
Rpim | 0.050 | 0.016 | 0.269 |
Number of reflections | 71664 | 3854 | 3512 |
<I/σ(I)> | 3.5 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 13.3 | 12.3 | 13.3 |
CC(1/2) | 0.998 | 0.833 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | 6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCL2, pH 6.7 |