7DAH
Adenosine triphosphate phosphoribosyltransferase from Vibrio cholerae in complex with ATP and PRPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-07-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 |
| Unit cell lengths | 136.603, 136.603, 121.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.320 - 2.920 |
| R-factor | 0.2186 |
| Rwork | 0.216 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.773 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.020 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Number of reflections | 55130 | 5579 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.992 | 0.497 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | 0.1M Morpheus Amino acids , 0.1M Morpheus Buffer System 3 , 30 % v/v Morpheus Precipitant Mix 3 (condition H11 of Morpheus screen from Molecular Dimensions) |
